Biomedical Image Processing – II

Continuing from Biomedical Image Processing – I

Image Properties

Once an image is stored in digital format, it can be described by a number of different parameters. Some of the relevant parameters are briefly discussed here. The traditional convention for an image coordinate system is depicted in Figure 4.

image-coordinate-system

Figure 4. General convention for image coordinate systems.

While biomedical images are generally viewed in 2D, it is sometimes helpful to view gray-scale (monochrome) images in perspective, with the third axis being brightness. This is illustrated with “pseudoimage” data in Figure 5.

pseudo-image-data

Figure 5. Pseudoimage data viewed in 2D and perspective modes. Data values (either a “0” or a “2”) are placed within a 31-row by 24-column matrix.

Important image parameters

Images can be described by a large number of different parameters. Some of these are listed here.

  • Pixels (a.k.a. picture elements, pels, image elements) – Individual rectangular
    elements that comprise an image. The term voxel describes a pixel’s 3D analog.
  • Gray levels – An 8-bit, gray-scale image with 1024 × 1024 pixels requires a
    megabyte of storage.
  • Color depth – Usually reported as powers of 2, this can range from 2 colors up to 32-
    bit color (a.k.a. True Color). Colors are often defined using RGB (red-green-blue) or
    HSB (hue-saturation-brightness) combinations (see Figure 6).
  • Aspect ratio – The scaling ratio between the x and y axes.
  • Contrast – The relationship between the brightest and dimmest pixel in the image.
  • Histogram – A binned representation of the gray levels, colors, or brightness levels
    in the image.

Other notes:

  • Because of the optimization properties of 2D Fourier transforms, it is often
    advantageous to select image sizes whose number of rows and columns are both
    powers of 2 (e.g., 512 x 512 array with 128 gray levels – comparable to a
    monochrome TV image)
  • In motion videos, images are displayed at a rate of 30 frames per second

RGB

rgb

Primary colors: red, green, blue
Secondary colors
: yellow = red + green, cyan = green +
blue, magenta = blue + red.

White = red + green + blue

Black = no light.
[http://www.cecs.csulb.edu/~jewett/colors/rgb.html]

HUE: actual color
Measured in angular degrees around the cone starting and ending at red = 0 or 360 (so yellow = 60, green = 120, etc.).

hue
SATURATION
: purity of the color
Measured in percent from the center of the cone (0) to the surface (100). At 0% saturation, hue is meaningless

BRIGHTNESS: measured in percent from black (0) to white (100). At 0% brightness, both hue and saturation are meaningless.
[http://www.cecs.csulb.edu/~jewett/colors/hsb.html]

Figure 6. RGB and HSB color descriptions.

The next Biomedical Image Processing lesson will discuss image analysis, classification, and component labeling.  Happy Holidays!

-Jeff

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